High-Throughput Plant Solutions: Bioreactors
BioF-IV High-Throughput Plant Bioreactor
Introduction
TheBioF-IVHigh-ThroughputPlantBioreactor representsthepinnacleof innovation inplant tissuecultureandmicropropagation technology.Designed to meet the needs of bothresearchersandindustrialusers,this system offers unparalleled efficiency, scalability,andeaseofuse.


Product Structure and Components
·High-Throughput Plant Bioreactor Composition:The bioreactor iscomposed of tank assemblies, series/parallel connection components,gas exchange and power control units,and inoculation accessories. ·Enhanced CultiationCapacity:Byconfiguring the reactor tanks in series or parall, the cultivation capacityof the plant bioreactor can be significantly increased, allowing for higher processing throughput. ·Integrated Design:The system features a holistic design with complete supporting components, ensuring ease of operation, effective contamination prevention, and improved inoculation efficiency
ApplicationFeatures
·Rapid Seedling Development:Significantly accelerates seedling growth, reducing culivation time by over one-third.
·Minimal Transfers Required:Eliminates or greatly reduces the need for transfers, cuting labor costs by more than half.
·Healthy and Robust Seedlings:Produces larger and stronger seedlings with synchronized acclimatization and probiotictreatment,leading tohighertransplantsurvival rates.
·VersatilCultivationCapabilties:Capable f cultivating celclusters, plant tissues, protocorms,hairyrots and other plant organs.
· Direct Development of Storage Organs:Extended cultivation allows direct development into tubers and bulbs, enablingtissue-culturedplantstoreachthemarketearlier.
· Plant-Microbe Co-Cultivation:Supports co-cultivation of microorganisms and plant tissues, enhancing the yied of natural products.
Product Advantages
·High Throughput:Dozens or even hundreds of plantlets can be cultivated within a single reactor vessel, significantly increasing the quantity compared to traditional tissue culture bottles.
·Simplified Process:The entirelifecycle or extended cultivation can beachieved without the need formultiple transfers,without affecting tissue development or plant growth.
● High Eficiency:Timely exchange of culture medium and gas ensures the supply of nutrients and \mathsf{C O}_{2}, enhancing growth efficiency.
· Cost Reduction:Saves a significant amount of materials, simplifies the inoculation process, and improves automation levels, thereby reducing labor costs.
·High Quality:lntermittent immersion cultiation reduces vitrification, resulting inhigh-quality seedlings and somatic embryos with high transplantation survival rates.
·Wide Applications:uitable for plant virus elimination, seeding propagation, breeding research,biprodution germplasm resource conservation, genetic transformation,and other fields.
Product Applications
MainCultivationObjectives:
·Propagationof Economic Crops andMedicinal PlantSeedlings:Strawberries,konjac,bananas,medicinal plants, flowers, etc.
·Breeding of SaltTolerant, rought-Resistant, and Disease-Resistant Varieties:Mutant screening and mass propagation and evaluation of progeny from individual plants.
·Large-Scale Cultivation of Plant Tisues, Cells, and Organs:Protocorms, bulbs, micro-tubers, artificial seeds.
·Acclimatization and Group Immunity of Seedlings:Concentrated treatment of steril tissue-cultured seedlings with probiotics and antagonistic bacteria during the late cultivation stage.
·Cell Factories and Product Preparation:Achieving high-level and stable expresson of active products through stress and co-cultivation.

Applied toSeedlingProduction
MassPropagationof Seedlings
Advantages in Cultivating Woody Plants:Overcoming browning and inhibition phenomena, enabling continuous growthordirectcuttingpropagation.

Cultivation of Tubers and Pseudobulbs asPropagules
By supplementing nutrients and extending the cultivation period in the bioreactor, propagules suchastubers can be further obtained, reducing the field growth time of tissue-cultured seedlings by approximately one year.
CultivationofProtocorms andOtherSterilePropagativeOrgans
The image below shows: Protocorms of Dendrobium nobile, which can express dendrobine and can also be induced to develop into Dendrobium seedlings.

CultivationofMedicinalPlantHairyRootsand Product Preparation
Theimagebelowshows:HairyrootsofLithospermum erythrorhizon,whichcanproducepharmaceuticals, cosmetics,and food additives.


Applied to Germplasm Resource Exploration and Cultivation
·Plantdetoxification and germplasmresource purification.
·Functional verification,transgenic,and molecular breeding of plant genes.
·Seedlingexpansionandindustrializedagriculture.
·Researchon plant mutants and heterozygous breeding.
·Plantcelldifferentiationandplantdevelopmental physiology.
·Plant cell totipotence
AppliedtoCutting,Grafting,andResourceConservation
The image below shows: The cultivation effects of tea seedlings and agarwood directly cut and cultured in the BioF-IVPlant Bioreactor.The portable reactor vessels offer large storage capacity, ease of transport, and convenient real-time observation and processing.

Applied to High-Throughput Variety Screening
ScreeningProcess and Cultivation Effectsof Salt-TolerantPinellia ternata Strains

AppliedtoControlledPollinationandFull LifeCycleStudies
The image shows: Promoting Dendrobium flowering and achieving artificial pollination through suficient nutrient supply in the BioF-IVPlant Bioreactor.Afterfullcultivation, anadequate amount of freshDendrobium stems is also obtained.

AppliedtoPlant/MicrobeCo-CultivationSystems
Applied to Group Immunity of Tissue-Cultured Seedlings and High-Efficiency Screening of Dominant MicrobialStrainsforBiofertilizers
Applied toBasic Research
AppliedtoPlantTissueand Stem Cell Cultivation

Applied to theResponse of Plant Tissues and Plants to Other Treatment Technologies

Studying theEffectsofEffectorsonPlantGrowth

Images:
CultivationprocessofDendrobiumnobile
tissue-cultured seedlings in the BioF-IV Bioreactor(a-j, each interval 15 days).
Effects ofdifferent immersionfrequenciesonseedling growthduringDendrobiumnobilecultivationinthe BioF-IV Bioreactor (third row).
Promotion/inhibitioneffectsofdifferentconcentrations of MeJAtreatmentonthegrowthofDendrobiumnobile cultivated in the bioreactor (fourthrow). EffectsofdifferentconcentrationsofMeJAstress treatmentonpromotingthelodgingofPinelliaternata in the bioreactor (fifth row).